mcherry激發波長是多少？

mcherry激發波長是多少？

mCherry是一種來自于蘑菇珊瑚（mushroom coral）的紅色熒光蛋白，常有于標記和示蹤某些分子和細胞組分。相對于其他熒光，mcherry的好處在于它的顏色和應用最多的GFP.html' target='_blank' title='綠色熒光蛋白' >綠色熒光蛋白（GFP）能進行共同標記，并且mcherry相對于其他單體熒光蛋白來說也具有卓越的光穩定型。

吸收和發射波長：

mcherry的更大吸收/發射峰分別位于587nm和610nm，對光致漂白耐受，熒光非常穩定。

常見應用：
?mCherry常用于與目的基因組成融合蛋白以及通過IRES或2A與感興趣的蛋白共表達；
?啟動子活性研究；
?熒光共振能量轉移(fluorescence resonance energy transfer，FRET）和其他定量實驗；
?標記細胞或者分子，進行示蹤實驗；

 mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria.

 
m前綴的意義：
mCherry的m為monmer單體的縮寫，表示mCherry熒光蛋白的形式為單體，這在很多實驗設計中非常重要，比如與目的標記基因組成融合蛋白時。

成熟時間：
mCherry具有較快的成熟速度，t0.5為15分鐘，這在一些需要做出快速反應的實驗非常重要。比如啟動子活性報告系統等。

如何觀察和篩選mcherry在植物中的表達？

你可以選用LUYOR-3415RG和LUYOR-3260GR便攜式熒光蛋白激發光源來直接觀察和篩選mcherry在植物中有沒有表達。如需進一步了解，請撥打電話153-1756-5658進行咨詢（或直接添加微信15317565658咨詢）。

mcherry紅色熒光蛋白的激發波長和發射波長

上圖為LUYOR-3415RG便攜式紅色熒光蛋白激發光源

上圖為LUV-50A紅色熒光蛋白觀察眼鏡

上圖為LUV-590A 紅色熒光蛋白拍照濾鏡

上圖為紅色熒光蛋白在大豆根系上的表達（LUYOR-3415RG照射，LUV-590A濾鏡拍攝）

實驗室常見熒光蛋白的激發波長和發射波長

紫外熒光蛋白UV Proteins

藍色熒光蛋白Blue Proteins

青色熒光蛋白Cyan Proteins

綠色熒光蛋白Green Proteins

黃色熒光蛋白Yellow Proteins

桔色熒光蛋白Orange Proteins

紅色熒光蛋白Red Proteins

遠紅熒光蛋白Far Red Proteins

近紅熒光蛋白Near IR Proteins

Sapphire-type Proteins

Red Fluorescent Proteins

Red fluorescent proteins (RFP) can be imaged on existing confocal or widefield microscopes, and they also have more penetrating power. The excitation and emission maxima of RFP are 558nm and 583 nm, respectively.

The use of RFP, however, has been hampered with several issues. RFP is an obligate tetramer - thus, it forms large aggregates inside cells. This makes the use to RFP to report the location of a protein severely limited.

Although GFP can successfully fuse with several hundreds of proteins, RFP-conjugated proteins are often toxic. Some variants of RFP have overcome these limitations. For example, DsRed2 fluorescent protein does not form aggregates and has reduced toxicity, while another variant of RFP (known as RedStar) has increased brightness and maturation rate.