YFP激發光波長和YFP的發射波長是多少？

YFP激發波長為510nm，更大發射波長為527 nm

黃色熒光蛋白（Yellow Fluorescent Protein ，YFP）可以看做GFP.html' target='_blank' title='綠色熒光蛋白' >綠色熒光蛋白的一種突變體，最初來源于維多利亞多管水母（ Aequorea victoria）。相對于綠色熒光蛋白，其熒光向紅色光譜偏移，而這主要是由于蛋白203位蘇氨酸變為酪氨酸。其更大激發波長為514 nm，更大發射波長為527 nm。

美國路陽生產有多款便攜式激發光源，無需熒光顯微鏡就能輕松觀察篩選黃色熒光蛋白表達陽性。便攜式YFP激發光源能直接分選YFP表達陽性的植株，能夠分選愈傷組織、植株、種子、菌落等。如欲進一步了解便攜式熒光蛋白激發光源，可直接聯系上海路陽生物技術有限公司的銷售客服。

上圖為黃色熒光蛋白激發光源

上圖為LUV-40A黃色熒光蛋白觀察眼鏡

上圖為LUV-550A 黃色熒光蛋白拍照濾鏡

Yellow Fluorescent Proteins/常用黃色熒光蛋白的激發波長和發射波長

Yellow Fluorescent Proteins

Yellow fluorescent protein (YFP) is a mutant variant of GFP. In this case, a mutation was introduced after the discovery that threonine residue was present near the chromophore in GFP.

To bring stability to the excited dipole moment of chromophore, this threonine residue was mutated. This led to shift by 20 nm in the excitation and emission wavelength of the fluorescent protein, leading to longer wavelengths.

YFP was further improved that led to development of enhanced yellow fluorescent protein or eYFP. This is one of the brightest fluorescent proteins and is widely used for different imaging purposes. GFP and YFP are used in several cases to perform dual imaging, which is a procedure where two molecules are tagged to two different fluorescent proteins. Consequently, they can be imaged together in order to visualize and appraise their inter-dynamics.

 黃色熒光蛋白的激發波長和發射波長